Biomarcador Cérebro Geral Imunohistoquímica

Subtyping of gliomas of various WHO grades by the application of immunohistochemistry

Escrito por Carla Brito Lopes

Subtyping of gliomas of various WHO grades by the application of immunohistochemistry

Aims
In 2010, four subtypes (classical, proneural, mesenchymal, and neural) of glioblastoma multiforme (GBM) were defined by molecular genetic analyses. The objective of this study was to assess whether gliomas, independently of the type and grade, could be subdivided into protein-based subtypes.

Methods and results
A tissue microarray (TMA) approach was applied to incorporate tissue samples of low-grade and high-grade gliomas into five TMAs. High expression levels of epidermal growth factor receptor (EGFR), CD44, c-MER proto-oncogene tyrosine kinase (MERTK), platelet-derived growth factor receptor α, p53, oligodendrocyte transcription factor 2 (OLIG2) and isocitrate dehydrogenase 1 with the R132H mutation were assessed using immunohistochemistry (IHC). Glioma could be subdivided into four subtypes by IHC. The majority of the low-grade gliomas were of the proneural subtype, i.e. high p53 expression (63% of grade II). The classical subtype, with high EGFR and low p53 expression, was most common in GBMs (39%), followed by the proneural (29%) and mesenchymal (with high CD44 and MERTK expression) (29%) subtypes, a frequency that is in line with previously published data based on molecular genetics.

Conclusions
Assessment of the expression of the five proteins EGFR, CD44, MERTK, p53 and OLIG2 is sufficient for subtyping gliomas, and can be recommended for implementation in clinical practice for both low-grade and high-grade gliomas.

 

Article first published online: 5 NOV 2013
DOI: 10.1111/his.12252
© 2013 John Wiley & Sons Ltd

Pode descarregar o artigo completo aqui: Subtyping of gliomas of various WHO grades by the application of immunohistochemistry


Citation: [Popova S N, Bergqvist M, Dimberg A, Edqvist P-H, Ekman S, Hesselager G, Ponten F, Smits A,Sooman L & Alafuzoff I (2013) Histopathology Subtyping of gliomas of various WHO grades by the application of immunohistochemistry 64, 365–379]. Reprinted with permission under the terms of the Creative Commons Attribution License.

Sobre o autor

Carla Brito Lopes

Carla nasceu a 14 de Fevereiro de 1977 em Viana do Castelo. Completou o bacharelato em Anatomia Patológica, Citológica e Tanatológica na ESTES-Porto em 1998 e licenciou-se em 2001 pela Escola Superior de Tecnologia da Saúde em Lisboa. Concluiu a certificação em Laboratory Management pela ASCP (American Association of Clinical Pathology) em Setembro de 2016.
Actualmente encontra-se a frequentar mestrado em Genética Molecular e Biomedicina na Faculdade de Ciências e Tecnologia da Universidade Nova de Lisboa.