Citologia Geral Imunohistoquímica

The usefulness of the cell transfer technique for immunocytochemistry of fine-needle aspirates.

Escrito por Carla Brito Lopes

BACKGROUND: The use of immunocytochemistry as an important ancillary technique for the workup of fine-needle aspiration (FNA) specimens is an important component in the daily practice of cytopathology. Although cell block preparations are commonly used, immunocytochemistry cannot be completed if the cell blocks are hypocellular. As an alternative approach when conventional cell blocks fail, immunocytochemistry can be performed on cell-transferred direct smears. Multiple immunostains can be applied to a single direct smear using the cell transfer technique (CTT).

METHODS: A retrospective review of all FNA cases performed at the study institution over a 3-year period was conducted. All cases in which CTT was used for immunocytochemistry were identified. The contribution of CTT to the final diagnosis in these cases was assessed.

RESULTS: CTT was used in 152 of the 11,259 FNAs performed. A total of 431 immunocytochemistry stains, including 46 different antibodies, were performed using CTT. In 118 of 150 cases, the immunocytochemistry results contributed significantly to the final diagnosis. It provided no added value in the remaining 32 cases and failed in 2 cases.

CONCLUSIONS: CTT is a very useful tool for the workup and diagnosis of FNA specimens when conventional cell blocks lack adequate cellularity. Although CTT was used in only 1.4% of FNA cases in the current study, it contributed to the final diagnosis in 79% of the cases in which it was used. For these patients, CTT reduced the need for repeat FNAs, thereby reducing potential patient morbidity and health care costs.

 

Keywords: cell transfer; cytology; fine-needle aspiration (FNA); immunocytochemistry

 

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Article first published: 30 JUL 2014

DOI: 10.1002/cncy.21469

Citation: Marshall, A. E., Cramer, H. M. and Wu, H. H. (2014), The usefulness of the cell transfer technique for immunocytochemistry of fine-needle aspirates. Cancer Cytopathology.

Copyright: © 2014 American Cancer Society.

From: US National Library of Medicine <http://www.nlm.nih.gov/> – National
Institutes of Health <http://www.nih.gov/>. http://www.ncbi.nlm.nih.gov/pubmed/25078555.
Disclaimer: http://www.ncbi.nlm.nih.gov/About/disclaimer.html

 

Sobre o autor

Carla Brito Lopes

Carla nasceu a 14 de Fevereiro de 1977 em Viana do Castelo. Completou o bacharelato em Anatomia Patológica, Citológica e Tanatológica na ESTES-Porto em 1998 e licenciou-se em 2001 pela Escola Superior de Tecnologia da Saúde em Lisboa. Concluiu a certificação em Laboratory Management pela ASCP (American Association of Clinical Pathology) em Setembro de 2016.
Actualmente encontra-se a frequentar mestrado em Genética Molecular e Biomedicina na Faculdade de Ciências e Tecnologia da Universidade Nova de Lisboa.